Reverse transcriptase

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HIV-1 reverse transcriptase P66 subunit (grey) and P51 subunit (green) complex with sulfate 3dlk

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Crystal Structure of unliganded HIV-1 Reverse transcriptase, 1hmv

Introduction

Reverse transcriptase (RT) or RNA-dependent DNA polymerase transcribes single-stranded RNA into double-stranded DNA. HIV-1 RT is from the human immunodeficiency virus and is a heterodimer of P66 and P51 subchains. The images at the left and at the right correspond to one representative RT structure, i.e. crystal structure of HIV-1 Reverse transcriptase (1hmv). P15 is its RNAse H domain. There are two types of inhibitors for RT: NNRTIs are the non-nucleoside inhibitors, and NRTIs are the nucleoide inhibitors. Being the protein that gives their name to Retroviruses, Reverse Transcriptase is, along with Protease and Integrase, the most important part of the protein system involved in the process of infection and reproduction for viruses like HIV, MuLV and AMV. RT has the unusual property of transcribing ssRNA into dsDNA going against the Central Dogma of Molecular Biology. Since its discovery in 1970, the study of its properties and mechanisms of action have been of high interest among the scientific community due to the unique properties that makes it an important medical target enzyme and important tool for genetic engineering applications like RT-PCR in the construction of cDNA libraries. See also

Reverse Transcriptase is one of the CBI Molecules being studied in the University of Massachusetts Amherst Chemistry-Biology Interface Program at UMass Amherst and on display at the Molecular Playground. -- CBI Molecular Playground Model --

Structure

This hand-like heterodimer protein has an usual length of 1000 residues (560 in Chain A and 440 for B), a third of them involved in alpha helices and almost a quarter involved in beta sheets, showing α+β secondary structure domains. Chain A has an usual weight of 66KDa whereas Chain B is around 51KDa. These monomers are derived from the same gene, but p51 lacks the amino acids of one active site and has a different tertiary structure conformation compared to p66. Because of this, p51 is enzymatically inactive^[1]. There are five distinct structures within the p66 subchain that are used to describe the functions of RT: the fingers (residues 1–85 and 118–155), the palm (residues 86–117 and 156–236), the thumb (residues 237–318), the connection (319–426), and the RNase H (residues 427-end). The palm contains the main active site (residues 110, 185-186)^[2].

Function

As a RNA-dependent DNA Polymerase, Reverse Transcriptase is able to recognize the initial RNA, transcribe it to ssDNA, cleave the remaining RNA and then build up the dsDNA. To do this the protein has two active catalytic zones. Chain A has the Polymerase active site that consist of two finger-like domains: one of them recognizes the initial nucleic acid by h-bond interactions with phosphate groups of the side chains, then both domains make a conformational change closing the recognition hole to allow the second domain with the support a Magnesium ion coordination system to begin the transcription process adding the specific DNA nucleotides. This change is allowed by a flexible zone between the two previous domains; it is used as a common pharmaceutical target site in order to prevent the change and therefore inhibit activity. This zone is the only zone of Chain A that has non-conserved aminoacids, giving the virus more drug resistance^[3] Link to Consurf Data Base for PDB Entry: 1JLB.

As the same rate that the polymerization process occurs, the other active site known as the Ribonuclease H domain cleaves RNA, releasing the ssDNA that comes again through the Polymerase active site to become dsDNA (all this with a second Magnesium coordinative system, that allows non-specific recognition, just with phosphates). Finally, Chain B, despite the similar aminoacid sequence with Chain A, has no enzymatic activity; its function is possibly to stabilize and interact with both active sites by varying the length between them in order to synchronize both functions.

This is the most general idea of the mechanism of action of Reverse Transcriptase; however the process remains unclear and new approaches are being reported ^[4].

One of the principal issues about this protein compared to usual DNA polymerase (besides to the similarity with the Klenow fragment), is the lack of a correction mechanism (usually made by DNA PolIII in the DNA Polymerase); this deficiency increases the number of errors, producing more mutations and therefore giving more facultative and resistance ability to the virus.

↑ Kohlstaedt LA, Wang J, Friedman JM, Rice PA, Steitz TA. Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science. 1992 Jun 26;256(5065):1783-90. PMID:1377403 doi:[https://dx.doi.org/10.1126/science.1377403 https://dx.doi.org/10.1126/science.1377403
↑ Sarafianos SG, Marchand B, Das K, Himmel DM, Parniak MA, Hughes SH, Arnold E. Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition. J Mol Biol. 2009 Jan 23;385(3):693-713. doi: 10.1016/j.jmb.2008.10.071. Epub 2008, Nov 3. PMID:19022262 doi:https://dx.doi.org/10.1016/j.jmb.2008.10.071
↑ ConSurf: Using Evolutionary Data to Raise Testable Hypotheses about Protein Function DOI: 10.1002/ijch.201200096
↑ Abbondanzieri EA, Bokinsky G, Rausch JW, Zhang JX, Le Grice SF, Zhuang X. Dynamic binding orientations direct activity of HIV reverse transcriptase. Nature. 2008 May 8;453(7192):184-9. PMID:18464735 doi:10.1038/nature06941

3D Structures of Reverse transcriptase

Updated on 07-January-2018

- 2b2a - TtRT telomerase catalytic subunit (mutant) - Tetrahymena thermophila
- 2r4g - TtRT telomerase catalytic subunit
- 5c9h - TtRT catalytic subunit + DNA
- 3du6, 3du5 - TcRT telomerase catalytic subunit - Tribolium castaneum
- 3kyl - TcRT telomerase catalytic subunit + DNA
- 4o26 – RT RNA-binding domain + RNA – rice fish
- 4lmo - RT telomerase RNA-binding domain – tiger puffer
- 5lgf - RT N-terminal - Ogataea polymorpha - NMR
- 5ugw - hRT thumb domain
- 5cqg - RT + inhibitor - red flour beetle

References

Proteopedia Page Contributors and Editors (what is this?)

Daniel Moyano-Marino, Alexander Berchansky, Eric Martz, Amol Kapoor, Lynmarie K Thompson, David Canner, Brian Foley, Jaime Prilusky, Joel L. Sussman, Michal Harel

[1] Kohlstaedt LA, Wang J, Friedman JM, Rice PA, Steitz TA. Crystal structure at 3.5 A resolution of HIV-1 reverse transcriptase complexed with an inhibitor. Science. 1992 Jun 26;256(5065):1783-90. PMID:1377403 doi:[https://dx.doi.org/10.1126/science.1377403 https://dx.doi.org/10.1126/science.1377403

[2] Sarafianos SG, Marchand B, Das K, Himmel DM, Parniak MA, Hughes SH, Arnold E. Structure and function of HIV-1 reverse transcriptase: molecular mechanisms of polymerization and inhibition. J Mol Biol. 2009 Jan 23;385(3):693-713. doi: 10.1016/j.jmb.2008.10.071. Epub 2008, Nov 3. PMID:19022262 doi:https://dx.doi.org/10.1016/j.jmb.2008.10.071

[3] ConSurf: Using Evolutionary Data to Raise Testable Hypotheses about Protein Function DOI: 10.1002/ijch.201200096

[4] Abbondanzieri EA, Bokinsky G, Rausch JW, Zhang JX, Le Grice SF, Zhuang X. Dynamic binding orientations direct activity of HIV reverse transcriptase. Nature. 2008 May 8;453(7192):184-9. PMID:18464735 doi:10.1038/nature06941

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Reverse transcriptase

Introduction

Structure

Function

3D Structures of Reverse transcriptase

See Also

References

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