5yuc | pdb_00005yuc

Crystal structure of voltage-gated sodium channel NavAb N49K mutant

spinqualitylabelsall models 5yuc, resolution 3.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5yuc is a 1 chain structure with sequence from Atcc 49616. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 1N7, CA, LMT Gene: SAMEA4475700_01771 (ATCC 49616) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Voltage-gated sodium channels are crucial for electro-signalling in living systems. Analysis of the molecular mechanism requires both fine electrophysiological evaluation and high-resolution channel structures. Here, we optimized a dual expression system of NavAb, which is a well-established standard of prokaryotic voltage-gated sodium channels, for E. coli and insect cells using a single plasmid vector to analyse high-resolution protein structures and measure large ionic currents. Using this expression system, we evaluated the voltage dependence and determined the crystal structures of NavAb wild-type and two mutants, E32Q and N49K, whose voltage dependence were positively shifted and essential interactions were lost in voltage sensor domain. The structural and functional comparison elucidated the molecular mechanisms of the voltage dependence of prokaryotic voltage-gated sodium channels. Optimized expression and purification of NavAb provide the structural insight into the voltage dependence.,Irie K, Haga Y, Shimomura T, Fujiyoshi Y FEBS Lett. 2017 Dec 22. doi: 10.1002/1873-3468.12955. PMID:29274127 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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