4c5a | pdb_00004c5a

The X-ray crystal structures of D-alanyl-D-alanine ligase in complex ADP and D-cycloserine phosphate

spinqualitylabelsall models 4c5a, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4c5a is a 3 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ADP, DS0, GOL, MG Related: 4c5b, 4c5c Activity: D-alanine--D-alanine ligase, with EC number 6.3.2.4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [C4ZRI7_ECOBW] Cell wall formation.[HAMAP-Rule:MF_00047][SAAS:SAAS00058712] Publication Abstract from PubMed D-cycloserine is an antibiotic which targets sequential bacterial cell wall peptidoglycan biosynthesis enzymes: alanine racemase and D-alanine:D-alanine ligase. By a combination of structural, chemical and mechanistic studies here we show that the inhibition of D-alanine:D-alanine ligase by the antibiotic D-cycloserine proceeds via a distinct phosphorylated form of the drug. This mechanistic insight reveals a bimodal mechanism of action for a single antibiotic on different enzyme targets and has significance for the design of future inhibitor molecules based on this chemical structure. Inhibition of D-Ala:D-Ala ligase through a phosphorylated form of the antibiotic D-cycloserine.,Batson S, de Chiara C, Majce V, Lloyd AJ, Gobec S, Rea D, Fulop V, Thoroughgood CW, Simmons KJ, Dowson CG, Fishwick CWG, de Carvalho LPS, Roper DI Nat Commun. 2017 Dec 5;8(1):1939. doi: 10.1038/s41467-017-02118-7. PMID:29208891 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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