1l3x | pdb_00001l3x
Solution Structure of Novel Disintegrin Salmosin
Structural highlights
Function[VM2HS_GLOBR] Metalloproteinase hxl-1: snake venom metalloproteinase that impairs hemostasis in the envenomed animal (By similarity).[1] [2] [3] [4] [5] Disintegrin salmosin-1: inhibits GPIIb/GPIIIa (ITGA2B/ITGB3) binding to immobilized fibrinogen with an IC(50) of 2.2 nM and ADP-induced platelet aggregation with an IC(50) of 131 nM, respectively. Inhibits angiogenesis.[6] [7] [8] [9] [10] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedDisintegrins are potent inhibitors of both platelet aggregation and integrin-dependent cell adhesion. A new disintegrin, salmosin, isolated from the venom of the Korean snake Agkistrodon halys brevicaudus, has been characterized by mass spectrometry and NMR spectroscopy, and its in vitro biological activity has been assessed. The IC(50) value of the purified salmosin was determined to be 2.2 nM in an assay for the inhibition of glycoprotein IIb-IIIa/fibrinogen interaction. Salmosin also inhibited the bovine capillary endothelial cell proliferation induced by bFGF in a dose-dependent manner. The NMR solution structures were well converged with a root-mean-square deviation of 0.76 A for backbone atoms among the 20 lowest energy structures, except for the arginylglycylaspartic acid (RGD) loop. The structure revealed that the conserved RGD motif with an unusual finger shape is distal from the rigid core of the C-terminal domain. Furthermore, even though the RGD motif did not interact with the hydrophobic core of the protein, it was stabilized by a network of molecular contacts through a small antiparallel beta-sheet comprising residues of Ile46-Ala50 and Asp54-Tyr58. Last, the electrostatic charge distribution on the surface of salmosin differs dramatically from that of other disintegrin proteins in that there is a cluster of negatively charged residues in close proximity to the RGD loop. Solution structure of a novel disintegrin, salmosin, from Agkistrondon halys venom.,Shin J, Hong SY, Chung K, Kang I, Jang Y, Kim DS, Lee W Biochemistry. 2003 Dec 16;42(49):14408-15. PMID:14661951[11] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||||
