5o98 | pdb_00005o98

Binary complex of Catharanthus roseus Vitrosamine Synthase with NADP+

spinqualitylabelsall models 5o98, resolution 1.55Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5o98 is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAP Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Monoterpene indole alkaloids, a large class of plant natural products, derive from the biosynthetic intermediate strictosidine aglycone. Strictosidine aglycone, which can exist as a variety of isomers, can be reduced to form numerous different structures. We have discovered a short chain alcohol dehydrogenase (SDR) from monoterpene indole alkaloid producing plants (Catharanthus roseus and Rauvolfia serpentina) that reduce strictosidine aglycone and produce an alkaloid that does not correspond to any previously reported compound. Here we report the structural characterization of this product, which we have named vitrosamine, as well as the crystal structure of the SDR. This discovery highlights the structural versatility of the strictosidine aglycone biosynthetic intermediate and expands the range of enzymatic reactions that SDRs can catalyze. This discovery further highlights how a sequence-based gene mining discovery approach in plants can reveal cryptic chemistry that would not be uncovered by classical natural product chemistry approaches. Discovery of a short chain dehydrogenase from Catharanthus roseus that produces a novel monoterpene indole alkaloid.,Stavrinides A, Tatsis E, Dang TT, Caputi L, Stevenson C, Lawson D, Schneider B, O'Connor S Chembiochem. 2018 Feb 9. doi: 10.1002/cbic.201700621. PMID:29424954 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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