5wab | pdb_00005wab

Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase

spinqualitylabelsall models 5wab, resolution 2.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5wab is a 4 chain structure with sequence from Bifaa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL Gene: BAD_1194 (BIFAA) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Bifidobacterium is an important genus of probiotic bacteria colonizing the human gut. These bacteria can uptake oligosaccharides for the fermentative metabolism of hexoses and pentoses, producing lactate, acetate as well as short-chain fatty acids and propionate. These end-products are known to have important effects on human health. beta-glucosidases (EC 3.2.1.21) are pivotal enzymes for the metabolism and homeostasis of Bifidobacterium, since they hydrolyze small and soluble saccharides, typically producing glucose. Here we describe the cloning, expression, biochemical characterization and the first X-ray structure of a GH3 beta-glucosidase from the probiotic bacteria Bifidobacterium adolescentis (BaBgl3). The purified BaBgl3 showed a maximal activity at 45 degrees C and pH 6.5. Under the optimum conditions, BaBgl3 is highly active on 4-nitrophenyl-beta-d-glucopyranoside (pNPG) and, at a lesser degree, on 4-nitrophenyl-beta-d-xylopyranoside (pNPX, about 32% of the activity observed for pNPG). The 2.4A resolution crystal structure of BaBgl3 revealed a three-domain structure composed of a TIM barrel domain, which together with alpha/beta sandwich domain accommodate the active site and a third C-terminal fibronectin type III (FnIII) domain with unknown function. Modeling of the substrate in the active site indicates that an aspartate interacts with the hydroxyl group of the C6 present in pNPG but absent in pNPX, which explains the substrate preference. Finally, the enzyme is significantly stabilized by glycerol and galactose, resulting in considerable increase in the enzyme activity and its lifetime. The structural and biochemical studies presented here provide a deeper understanding of the molecular mechanisms of complex carbohydrates degradation utilized by probiotic bacteria as well as for the development of new prebiotic oligosaccharides. Structural and biochemical characterization of a GH3 Beta-glucosidase from the probiotic bacteria Bifidobacterium adolescentis.,Florindo RN, Souza VP, Manzine LR, Camilo CM, Marana SR, Polikarpov I, Nascimento AS Biochimie. 2018 Mar 16. pii: S0300-9084(18)30068-3. doi:, 10.1016/j.biochi.2018.03.007. PMID:29555372 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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