Eukaryotic initiation factor

spinqualitylabelsall models Human initiation factor EIF4E complex with 7-methyl GPPPA 1ipb Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Eukaryotic initiation factors (EIF) are involved in the initiation of protein translation. They form a complex with the small subunit of the ribosome and Met-tRNA which scans the mRNA and recognizes the initiation codon AUG. For details on EIF4 see C-terminal portion of human eIF4GI. Disease Overexpression of individual subunits of eIF3 may cause malignant transformation . Overexpression of eIF4g is implicated in breast cancer. Structural insights EIF domains include the N terminal domain (NTD), C terminal domain (CTD), RNA recognition domain, middle domain, PAZ domain of EIF2C and a protein-protein interaction domain (PCI) of EIF3. P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE (7-methyl GPPPA) binding site. Water molecules shown as red spheres.

3D structures of eukaryotic initiation factor

Updated on 17-May-2018

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• EIF1
  • C-terminal portion of human eIF4GI, 1d7q – hEIF1 – human – NMR
    
  • 2ogh - yEIF1 – yeast - NMR
    
  • 3j80, 3j81, 4uer – EIF1A in 40S ribosome – Kluyveromyces lactis – cryo EM
    
  • 4kzx, 4kzy, 4kzz – rEIF1A in 40S ribosome – rabbit – cryo EM
    
  • 3zjy – hEIF1A + importin 13 + RanGTP
    
• EIF2
  • 1kl9, 1q8k, 5oa9 – hEIF2A subunit 1
    
  • 1q8x – hEIF2A - NMR
    
  • 6ezo – hEIF2B + drug
    
  • 5w2f – hEIF2D C-terminal
    
  • 5oa3 – hEIF2D in 40S ribosome – Cryo EM
    
  • 1q46 – yEIF2A – yeast
    
  • 3wj9 - EIF2A – fission yeast
    
  • 2a19, 2a1a – yEIF2A (mutant) + interferon-induced protein kinase
    
  • 4zgq, 4zgn – yEIF2G residues 410-527 + CDC123
    
  • 3ecs – hEIF2B
• EIF2C or argonaute
  • 4kxt, 4krf, 4kre, 1si2, 1si3 – hEI2C1 + RNA
    
  • 5w6v – hEI2C1 + RNA + GW182
    
  • 3luk – hEI2C2 mid domain
    
  • 3lud, 3lug, 3luh, 3luj, 3qx8, 3qx9 - hEIF2C mid domain + nucleotide
    
  • 5t7b, 5ki6, 4f3t – hEI2C2 + RNA
    
  • 4z4i, 4z4h, 4z4g, 4z4f, 4z4e, 4z4d, 4z4c, 4w5t, 4w5r, 4w5q, 4w5o, 4w5n, 4ola, 6cbd, 5wea, 5js2, 5js1, 4ei3 – hEI2C2 (mutant) + RNA
    
  • 5vm9 – hEI2C3 + RNA
    
• EIF3
  • 4k51, 4u1d - yEIF3A PCI domain
    
  • 2nlw - hEIF3B RNA recognition domain – NMR
    
  • 3ns5, 3ns6 - yEIF3B RNA recognition domain
    
  • 4u1f - yEIF3B middle domain
    
  • 5h7u - yEIF3C residues 36-163
    
  • 2krb - hEIF3B RNA recognition domain + hEIF3J peptide
    
  • 5k1h – hEIF3B + EIF3A C-terminal
    
  • 4nox – CtEIF3B – Chaetomium thermophilum
    
  • 5krb, 5krc, 5krd, 5k4d, 5k4c, 5k4b – EIF3D – wasp
    
  • 2cq0 – hEIF3G RNA recognition domain – NMR
    
  • 2mjc - hEIF3G residues 153-176 – NMR
    
  • 5k0y - rEIF3G in ribosome – cryo EM
    
  • 3zwl - yEIF3I + EIF3B CTD
    
  • 4u1c - yEIF3A PCI + yEIF3C PCI
    
  • 4u1e - yEIF3B CTD + yEIF3I + yEIF3G NTD
    
  • 5a5u - rEIF3B + rEIF3I – Cryo EM
    
  • 5a5t - rEIF3A + rEIF3C + rEIF3E + rEIF3F + rEIF3H + rEIF3K + rEIF3M – Cryo EM
    
  • 3bpj – hEIF3J
    
  • 1rz4 – hEIF3K
    
  • 3j8b, 3j8c - hEIF3A + hEIF3C + hEIF3E + hEIF3F + hEIF3H + hEIF3K + hEIF3L + hEIF3M – cryo EM
    
• EIF4
  • 1fuk – yEIF4A CTD
    
  • 1fuu - yEIF4A mRNA helicase domain
    
  • 2g9n, 3bor – hEIF4A DEAD domain
    
  • 1wi8, 2j76 – hEIF4B RNA recognition domain - NMR
    
  • 2gpq, 3tf2 – hEIF4E
    
  • 5gw6 – hEIF4E (mutant)
    
  • 2wmc - EIF4E - pea
    
  • 5me7 - mmEIF4E - muskmelon
    
  • 2idr – wEIF4E – wheat
    
  • 3l6a - hEIF4GII CTD
    
  • 1ug3 – hEIF4GI CTD (mutant)
    
  • 1hu3, 3d3m, 4iul – hEIF4GII middle domain
    
  • 2dng – hEIF4H RNA recognition domain – NMR
    
• EIF4 complexes
  • 3ex7, 2xb2 – hEIF4A + MAGO homolog + RNA-binding protein 8A + CASC3 + RNA
    
  • 2zu6, 3eiq – hEIF4A + programmed cell death protein
    
  • 4c9b - hEIF4A + CWC22
    
  • 5mqf - hEIF4A in splicesome – Cryo EM
    
  • 5ekv - hEIF4E + 4E-BP1 peptide + inhibitor
    
  • 2jgb - hEIF4E + nucleotide + 4E-BP1 peptide
    
  • 4tpw, 4tqb, 4tqc - hEIF4E + nucleotide + inhibitor
    
  • 4bea - hEIF4E + stapled peptide
    
  • 5t46 - hEIF4E + EIF4GI
    
  • 5nvn - hEIF4E + EIF4E-binding protein
    
  • 5nvm, 5nvl, 5nvk - hEIF4E + GRB10-interacting GYF protein
    
  • 5ehc, 5ei3, 5eir - hEIF4E + EIF4GI + inhibitor
    
  • 1ej1, 1l8b, 4b6u, 4b6v - mEIF4E + nucleotide - mouse
    
  • 2idv - wEIF4E (mutant) + GDP
    
  • 1ipb, 1ipc - hEIF4E + nucleotide
    
  • 4dt6, 4dum – hEIF4E + inhibitor
    
  • 2w97, 4aza – hEIF4E + hEIF4G peptide
    
  • 2v8w, 2v8x, 2v8y, 3u7x, 2jgc - hEIF4E + EIF4E-BP1 peptide
    
  • 3am7, 1wkw - hEIF4E + EIF4E-BP1 peptide + nucleotide
    
  • 1ej4 - mEIF4E + EIF4E-BP1 peptide
    
  • 5osx, 5m84, 5m83, 5m81, 5m80, 5m7z, 5m7x, 5m7w, 5m7v, 5j5y, 5j5o - mEIF4E + mRNA cap analog
    
  • 5bxv, 1ejh - mEIF4E + nucleotide + 4E-BP1 peptide
    
  • 3hxg, 3hxi - EIF4E + EIF4E-BP1 peptide + GDP – Schistosoma mansoni
    
  • 3m93, 3m94 - EIF4E + EIF4E-BP1 peptide + nucleotide – Pig roundworm
    
  • 5abx, 5aby - EIF4E + EIF4E-BP1 peptide + nucleotide – roundworm
    
  • 1ap8 – yEIF4E + GDP – NMR
    
  • 1rf8 - yEIF4E + yEIF4GI peptide + GDP
    
  • 2vso, 2vsx - yEIF4E + yEIF4F
    
  • 4axg - DmEIF4E + protein CUP – Drosophila melanogaster
    
  • 4ue8, 4uea, 4ueb, 4ued, 5abu, 5abv - DmEIF4E + 4E-BP1
    
  • 4ue9 - DmEIF4E + 4E transporter
    
  • 4uec, 5t47, 5t48 - DmEIF4E + DmEIF4G
    
  • 5me6 - mmEIF4E + GDP derivative
    
  • 5me5 - mmEIF4E + EIF4GI
    
• EIF5
  • 1eif, 2eif – EIF5A – Methanocaldococcus janaschii
    
  • 1x6o – EIF5A – Leishmania braziliensis
    
  • 1xtd - EIF5A – Leishmania mexicana
    
  • 3hks – EIF5A – Arabidopsis thaliana
    
  • 5hy6 – EIF5A – armywarm
    
  • 3cpf - hEIF
    
  • 2ful – yEIF5 CTD
    
  • 2iu1 - hEIF5 CTD
    
  • 2g2k – hEIF5 NTD (mutant) – NMR
    
  • 2e9h – hEIF5 – NMR
    
  • 3cpf – hEIF5A
    
  • 5dlq – hEIF5A + exportin 4 + GTPase RAN + GTP
    
  • 3er0 - yEIF5A
    
  • 3wbi, 3wbj, 4n3s – yEIF5B
    
  • 3wbk – yEIF5B + yEIF1A
    
  • 4ncf – yEIF5B + nucleotide
    
  • 4tmt, 4tmv, 4tmw, 4tmx, 4tmz, 4tn1 – CtEIF5B (mutant) + nucleotide
    
• EIF6
  • 1g62 – yEIF6
    
  • 2x7n – yEIF6S + sarcin-ricin loop + ribosomal protein L23 + ribosomal protein L24
    
  • 3j2i – yEIF6 + proliferation-associated protein 2G4 – cryo EM
    
  • 5m3q – CtEIF6
    
  • 5anb, 5an9 – EIF6 in 60S ribosome – slime mold – cryo EM
    

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Michal Harel, Alexander Berchansky, Joel L. Sussman