2xhs | pdb_00002xhs

Crystal structure of the ligand binding domain of Fushi tarazu factor 1 of Drosophila melanogaster.

spinqualitylabelsall models 2xhs, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2xhs is a 2 chain structure with sequence from Drome. This structure supersedes the now removed PDB entry 2iz2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: MSE Related: 1ftz, 2iz2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [FTZF1_DROME] Acts as a cofactor to fushi tarazu (ftz). Facilitates the binding of ftz to DNA. Binds the sequence element 5'-YCYYGGYCR-3' in the zebra element of ftz. Probably also functions as a receptor for a yet unknown ligand. [FTZ_DROME] May play a role in determining neuronal identity, may be directly involved in specifying identity of individual neurons. Required during embryogenesis for the process of body segmentation. Homeotic protein, required in alternating segment primordia, it specifies the correct number of segments. Publication Abstract from PubMed The interaction between the orphan nuclear receptor FTZ-F1 (Fushi tarazu factor 1) and the segmentation gene protein FTZ is critical for specifying alternate parasegments in the Drosophila embryo. Here, we have determined the structure of the FTZ-F1 ligand-binding domain (LBD).FTZ peptide complex using x-ray crystallography. Strikingly, the ligand-binding pocket of the FTZ-F1 LBD is completely occupied by helix 6 (H6) of the receptor, whereas the cofactor FTZ binds the co-activator cleft site of the FTZ-F1 LBD. Our findings suggest that H6 is essential for transcriptional activity of FTZ-F1; this is further supported by data from mutagenesis and activity assays. These data suggest that FTZ-F1 might belong to a novel class of ligand-independent nuclear receptors. Our findings are intriguing given that the highly homologous human steroidogenic factor-1 and liver receptor homolog-1 LBDs exhibit sizable ligand-binding pockets occupied by putative ligand molecules. Crystal structure of fushi tarazu factor 1 ligand binding domain/fushi tarazu Peptide complex identifies new class of nuclear receptors.,Yoo J, Ko S, Kim H, Sampson H, Yun JH, Choe KM, Chang I, Arrowsmith CH, Krause HM, Cho HS, Lee W J Biol Chem. 2011 Sep 9;286(36):31225-31. Epub 2011 Jul 20. PMID:21775434 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA