6fzb | pdb_00006fzb

AadA in complex with ATP, magnesium and streptomycin

spinqualitylabelsall models 6fzb, resolution 2.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6fzb is a 2 chain structure with sequence from Salty. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ATP, CL, GOL, MG, PEG, SRY Related: 5g4a, 5luh, 5lpa Gene: aadA, STM1264 (SALTY) Activity: -adenylyltransferase Streptomycin 3-adenylyltransferase, with EC number 2.7.7.47 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Streptomycin and spectinomycin are antibiotics that bind to the bacterial ribosome and perturb protein synthesis. The clinically most prevalent bacterial resistance mechanism is their chemical modification by aminoglycoside-modifying enzymes such as aminoglycoside nucleotidyltransferases (ANTs). AadA from Salmonella enterica is an aminoglycoside (3)(9) adenylyl transferase that O-adenylates position 3" of streptomycin and position 9 of spectinomycin. We previously reported the apo AadA structure with a closed active site. To clarify how AadA binds ATP and its two chemically distinct drug substrates, we here report crystal structures of wildtype AadA complexed with ATP, magnesium, and streptomycin and of an active-site mutant, E87Q, complexed with ATP and streptomycin or the closely related dihydrostreptomycin. These structures revealed that ATP binding induces a conformational change that positions the two domains for drug binding at the interdomain cleft and disclosed the interactions between both domains and the three rings of streptomycin. Spectinomycin docking followed by molecular dynamics simulations suggested that despite the limited structural similarities with streptomycin, spectinomycin makes similar interactions around the modification site, and, in agreement with mutational data, critically interacts with fewer residues. Using structure-guided sequence analyses of ANT(3")(9) enzymes acting on both substrates and ANT(9) enzymes active only on spectinomycin, we identified sequence determinants for activity on each substrate. We experimentally confirmed that Trp-173 and Asp-178 are essential only for streptomycin resistance. Activity assays indicated that Glu-87 is the catalytic base in AadA and that the non-adenylating E87Q mutant can hydrolyze ATP in the presence of streptomycin. Structural mechanism of AadA, a dual specificity aminoglycoside adenyl transferase from Salmonella enterica.,Stern AL, Van der Verren SE, Kanchugal P S, Nasvall J, Gutierrez de Teran H, Selmer M J Biol Chem. 2018 Jun 5. pii: RA118.003989. doi: 10.1074/jbc.RA118.003989. PMID:29871922 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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