6dch | pdb_00006dch

Structure of isonitrile biosynthesis enzyme ScoE

spinqualitylabelsall models 6dch, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6dch is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, CHT, CL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The electron-rich isonitrile is an important functionality in bioactive natural products, but its biosynthesis has been restricted to the IsnA family of isonitrile synthases. We here provide the first structural and biochemical evidence of an alternative mechanism for isonitrile formation. ScoE, a putative non-heme iron(II)-dependent enzyme from Streptomyces coeruleorubidus, was shown to catalyze the conversion of (R)-3-((carboxymethyl)amino)butanoic acid to (R)-3-isocyanobutanoic acid through an oxidative decarboxylation mechanism. This work further provides a revised scheme for the biosynthesis of a unique class of isonitrile lipopeptides, members of which are critical for the virulence of pathogenic mycobacteria. Isonitrile Formation by a Non-heme Iron(II)-Dependent Oxidase/Decarboxylase.,Harris N, Born D, Cai W, Huang Y, Martin J, Khalaf R, Drennan C, Zhang W Angew Chem Int Ed Engl. 2018 Jun 15. doi: 10.1002/anie.201804307. PMID:29906336 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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