6c7z | pdb_00006c7z

Crystal structure of the Q108K:K40L:T51V:R58F mutant of human Cellular Retinol Binding Protein II in complex with synthetic Ligand Julolidine

spinqualitylabelsall models 6c7z, resolution 1.42Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6c7z is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACT, JLO Related: 5f58 Gene: RBP2, CRBP2 (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [RET2_HUMAN] Intracellular transport of retinol. Publication Abstract from PubMed Mutants of human Cellular Retinol Binding Protein II (hCRBPII) were engineered to bind a julolidine retinal analog for the purpose of developing a ratiometric pH sensor. The design relied on the electrostatic influence of a titratable amino acid side chain, affecting the absorption, and thus emission of the protein/fluorophore complex. The ratio of emissions obtained at two excitation wavelengths that correspond to the absorption of the two forms of the protein/fluorophore complex, leads to a concentration independent measure of pH. A Genetically Encoded Ratiometric pH-Probe: Wavelength Regulation-Inspired Design of pH Indicators.,Berbasova T, Tahmasebi Nick S, Nosrati M, Nossoni Z, Santos E, Vasileiou C, Geiger J, Borhan B Chembiochem. 2018 Apr 12. doi: 10.1002/cbic.201800050. PMID:29645331 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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