6f2d | pdb_00006f2d

A FliPQR complex forms the core of the Salmonella type III secretion system export apparatus.

spinqualitylabelsall models 6f2d, resolution 4.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6f2d is a 10 chain structure with sequence from "bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: fliP, LTSERUB_0568 ("Bacillus cholerae-suis" Smith 1894), fliR, flaP, STM1981 ("Bacillus cholerae-suis" Smith 1894), fliQ, flaQ, STY2188, t0897 ("Bacillus cholerae-suis" Smith 1894) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [G5QE81_SALRU] Plays a role in the flagellum-specific transport system.[RuleBase:RU362069] [FLIQ_SALTI] Required for the assembly of the rivet at the earliest stage of flagellar biosynthesis. [FLIR_SALTY] Role in flagellar biosynthesis. Publication Abstract from PubMed Export of proteins through type III secretion systems is critical for motility and virulence of many major bacterial pathogens. Three putative integral membrane proteins (FliP, FliQ, FliR) are suggested to form the core of an export gate in the inner membrane, but their structure, assembly and location within the final nanomachine remain unclear. Here, we present the cryoelectron microscopy structure of the Salmonella Typhimurium FliP-FliQ-FliR complex at 4.2 A. None of the subunits adopt canonical integral membrane protein topologies, and common helix-turn-helix structural elements allow them to form a helical assembly with 5:4:1 stoichiometry. Fitting of the structure into reconstructions of intact secretion systems, combined with cross-linking, localize the export gate as a core component of the periplasmic portion of the machinery. This study thereby identifies the export gate as a key element of the secretion channel and implies that it primes the helical architecture of the components assembling downstream. Structure of the core of the type III secretion system export apparatus.,Kuhlen L, Abrusci P, Johnson S, Gault J, Deme J, Caesar J, Dietsche T, Mebrhatu MT, Ganief T, Macek B, Wagner S, Robinson CV, Lea SM Nat Struct Mol Biol. 2018 Jul;25(7):583-590. doi: 10.1038/s41594-018-0086-9. Epub, 2018 Jul 2. PMID:29967543 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA