6fly | pdb_00006fly

Structure of AcmJRL, a mannose binding jacalin related lectin from Ananas comosus, in complex with mannose.

spinqualitylabelsall models 6fly, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6fly is a 2 chain structure with sequence from Ananas comosus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MAN Related: 6flw Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A mannose binding jacalin-related lectin from Ananas comosus stem (AcmJRL) was purified and biochemically characterized. This lectin is homogeneous according to native, SDS-PAGE and N-terminal sequencing and the theoretical molecular mass was confirmed by ESI-Q-TOF-MS. AcmJRL was found homodimeric in solution by size-exclusion chromatography. Rat erythrocytes are agglutinated by AcmJRL while no agglutination activity is detected against rabbit and sheep erythrocytes. Hemagglutination activity was found more strongly inhibited by mannooligomannosides than by D-mannose. The carbohydrate-binding specificity of AcmJRL was determined in some detail by isothermal titration calorimetry. All sugars tested were found to bind with low affinity to AcmJRL, with Ka values in the mM range. In agreement with hemagglutination assays, the affinity increased from D-mannose to di-, tri- and penta-mannooligosaccharides. Moreover, the X-ray crystal structure of AcmJRL was obtained in an apo form as well as in complex with D-mannose and methyl-alpha-D-mannopyranoside, revealing two carbohydrate-binding sites per monomer similar to the banana lectin BanLec. The absence of a wall separating the two binding sites, the conformation of beta7beta8 loop and the hemagglutinating activity are reminiscent of the BanLec His84Thr mutant, which presents a strong anti-HIV activity in absence of mitogenic activity. Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem.,Azarkan M, Feller G, Vandenameele J, Herman R, El Mahyaoui R, Sauvage E, Vanden Broeck A, Matagne A, Charlier P, Kerff F Sci Rep. 2018 Jul 31;8(1):11508. doi: 10.1038/s41598-018-29439-x. PMID:30065388 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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