2oki | pdb_00002oki

Crystal structure of dimeric form of PfFabZ in crystal form2

spinqualitylabelsall models 2oki, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2oki is a 2 chain structure with sequence from Plafa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2okh, 1zhg, 1u1z, 1z6b Gene: fabz (PLAFA) Activity: [acyl-carrier-protein_dehydratase 3-hydroxyacyl-[acyl-carrier-protein] dehydratase], with EC number 4.2.1.59 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The crystals obtained from various batches of crystallization trials of FabZ from Plasmodium falciparum exhibited non-isomorphism. The c axis of the I222 cell showed a large variation of about 16 A, from c = 81 A to c = 97 A. Complete data sets were collected for three crystal forms with varying lengths of the c axis (form 1, c = 97 A; form 2, c = 92 A; form 3, c = 81 A). The crystal structure of form 1 has been reported previously. Here, the crystal structures of the other two crystal forms are reported and a detailed structural comparison is made of the three crystal forms in order to explore the possible reasons for the existence of non-isomorphism. The conformations of three loops vary between the three crystal forms. The disposition of the loops affects the crystal packing and hence the unit-cell parameter. The crystallization condition and crystallization method employed, which change the evaporation rate, determine the crystal form of the enzyme. The present analysis shows that pH-induced intrinsic conformational changes in the protein play a key role in the observed differences. Packing and loop-structure variations in non-isomorphous crystals of FabZ from Plasmodium falciparum.,Swarnamukhi PL, Sharma SK, Padala P, Surolia N, Surolia A, Suguna K Acta Crystallogr D Biol Crystallogr. 2007 Apr;63(Pt 4):458-64. Epub 2007, Mar 16. PMID:17372349 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Beta-hydroxyacyl-acyl carrier protein dehydratase

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