6dfe | pdb_00006dfe

The structure of a ternary complex of E. coli WaaC

spinqualitylabelsall models 6dfe, resolution 2.31Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6dfe is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GA7, GN1, GN4, KDO Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Heptosyltransferase I (WaaC) is a highly conserved glycosyltransferase found in Gram-negative bacteria that transfers a heptose residue onto the endotoxin inner core structure (ReLPS) of the outer membrane. Knockouts of WaaC have decreased virulence and increased susceptibility to antibiotics, making WaaC a potential drug target. While previous studies have elucidated the structure of the holoenzyme and a donor analog complex, no information on the binding mode of the acceptor has been available so far. By soaking of a chemically modified functional acceptor, along with a stable donor analog, the crystal structure of a pseudo-ternary complex of WaaC was obtained at 2.3-A resolution. The acceptor is bound in an unusual horseshoe conformation stabilized by interaction of the anionic carboxylate and phosphate groups at its center and tips with highly conserved Lys and Arg residues. This binding is accompanied by both inter- and intra-domain movements within the protein. Insights into Heptosyltransferase I Catalysis and Inhibition through the Structure of Its Ternary Complex.,Blaukopf M, Worrall L, Kosma P, Strynadka NCJ, Withers SG Structure. 2018 Jul 23. pii: S0969-2126(18)30247-8. doi:, 10.1016/j.str.2018.07.001. PMID:30122450 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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