2wqt | pdb_00002wqt

Dodecahedral assembly of MhpD

spinqualitylabelsall models 2wqt, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2wqt is a 20 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: K, NA, PO4 Related: 1sv6 Activity: 2-oxopent-4-enoate hydratase, with EC number 4.2.1.80 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The pentameric Escherichia coli enzyme 2-hydroxypentadienoic acid hydratase assembles to form a 20-nm-diameter particle comprising 60 protein subunits, arranged with 532 symmetry when crystallised at low pH in the presence of phosphate or sulphate ions. The particles form rapidly and are stable in solution during gel filtration at low pH. They are probably formed through trimers of pentamers, which are stabilised by the interaction of two phosphate ions with residues of the N-terminal domains of subunits at the 3-fold axis. Once the particles are formed at high concentrations of phosphate (or sulphate), they remain stable in solution at 20-fold lower concentrations of the anion. Guest molecules can be trapped within the hollow protein shell during assembly. The C-termini of the subunits are freely accessible on the surface of the protein cage and thus are ideal sites for addition of affinity tags or other modifications. These particles offer a convenient model system for studying the assembly of large symmetrical structures and a novel protein nanoparticle for encapsulation and cargo delivery. Assembly of a 20-nm protein cage by Escherichia coli 2-hydroxypentadienoic acid hydratase.,Montgomery MG, Coker AR, Taylor IA, Wood SP J Mol Biol. 2010 Mar 12;396(5):1379-91. Epub 2010 Jan 4. PMID:20053352 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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