2wpv | pdb_00002wpv
Crystal structure of S. cerevisiae Get4-Get5 complex
Structural highlights
Function[GET4_YEAST] May play a role in insertion of tail-anchored proteins into the endoplasmic reticulum membrane.[1] [MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.[2] [3] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedGet3, Get4 and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4/Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4/Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4 and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins. Crystal structure of Get4/Get5 complex and its interactions with Sgt2, Get3 and Ydj1.,Chang YW, Chuang YC, Ho YC, Cheng MY, Sun YJ, Hsiao CD, Wang C J Biol Chem. 2010 Jan 27. PMID:20106980[4] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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