2r8b | pdb_00002r8b
The crystal structure of the protein Atu2452 of unknown function from Agrobacterium tumefaciens str. C58
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| 2r8b, resolution 2.56Å | |||||||||||||
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| Ligands: | MSE, SO4 | ||||||||||||
| Gene: | AGR_C_4453, Atu2452 (Agrobacterium tumefaciens str. C58) | ||||||||||||
| Domains: | COG0400 | ||||||||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.
About this Structure
2R8B is a Single protein structure of sequence from Agrobacterium tumefaciens str. c58. Full crystallographic information is available from OCA.
Reference
In situ proteolysis for protein crystallization and structure determination., Dong A, Xu X, Edwards AM, Chang C, Chruszcz M, Cuff M, Cymborowski M, Di Leo R, Egorova O, Evdokimova E, Filippova E, Gu J, Guthrie J, Ignatchenko A, Joachimiak A, Klostermann N, Kim Y, Korniyenko Y, Minor W, Que Q, Savchenko A, Skarina T, Tan K, Yakunin A, Yee A, Yim V, Zhang R, Zheng H, Akutsu M, Arrowsmith C, Avvakumov GV, Bochkarev A, Dahlgren LG, Dhe-Paganon S, Dimov S, Dombrovski L, Finerty P Jr, Flodin S, Flores A, Graslund S, Hammerstrom M, Herman MD, Hong BS, Hui R, Johansson I, Liu Y, Nilsson M, Nedyalkova L, Nordlund P, Nyman T, Min J, Ouyang H, Park HW, Qi C, Rabeh W, Shen L, Shen Y, Sukumard D, Tempel W, Tong Y, Tresagues L, Vedadi M, Walker JR, Weigelt J, Welin M, Wu H, Xiao T, Zeng H, Zhu H, Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4. PMID:17982461
Page seeded by OCA on Mon Mar 31 04:57:37 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Agrobacterium tumefaciens str. c58
- Single protein
- Edwards, A M.
- Joachimiak, A.
- MCSG, Midwest Center for Structural Genomics.
- Savchenko, A.
- Tan, K.
- Xu, X.
- Zheng, H.
- Apc6088
- Mcsg
- Midwest center for structural genomic
- Protein atu2452
- Protein structure initiative
- Psi-2
- Structural genomic
- Unknown function
