4jj3 | pdb_00004jj3

Crystal structure of MamP soaked with iron(II)

spinqualitylabelsall models 4jj3, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4jj3 is a 2 chain structure with sequence from Magmm. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HEC Related: 4jj0 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Magnetotactic bacteria align along the Earth's magnetic field using an organelle called the magnetosome, a biomineralized magnetite (Fe(II)Fe(III)2O4) or greigite (Fe(II)Fe(III)2S4) crystal embedded in a lipid vesicle. Although the need for both iron(II) and iron(III) is clear, little is known about the biological mechanisms controlling their ratio. Here we present the structure of the magnetosome-associated protein MamP and find that it is built on a unique arrangement of a self-plugged PDZ domain fused to two magnetochrome domains, defining a new class of c-type cytochrome exclusively found in magnetotactic bacteria. Mutational analysis, enzyme kinetics, co-crystallization with iron(II) and an in vitro MamP-assisted magnetite production assay establish MamP as an iron oxidase that contributes to the formation of iron(III) ferrihydrite eventually required for magnetite crystal growth in vivo. These results demonstrate the molecular mechanisms of iron management taking place inside the magnetosome and highlight the role of magnetochrome in iron biomineralization. Structural insight into magnetochrome-mediated magnetite biomineralization.,Siponen MI, Legrand P, Widdrat M, Jones SR, Zhang WJ, Chang MC, Faivre D, Arnoux P, Pignol D Nature. 2013 Oct 31;502(7473):681-4. doi: 10.1038/nature12573. Epub 2013 Oct 6. PMID:24097349 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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