2v5p | pdb_00002v5p
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| 2v5p, resolution 4.10Å | |||||||||||||
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| Sites: | AC1, AC2, AC3, AC5, AC6, AC7 and AC8 | ||||||||||||
| Ligands: | BMA, NAG | ||||||||||||
| Related: | 1GF2, 1IGL, 1E6F, 1GP0, 1GP3, 1GQB, 1JPL, 1JWG, 1LF8, 2CNJ, 2V5N, 2V5O
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
COMPLEX STRUCTURE OF HUMAN IGF2R DOMAINS 11-13 BOUND TO IGF-II
Overview
Embryonic development and normal growth require exquisite control of insulin-like growth factors (IGFs). In mammals the extracellular region of the cation-independent mannose-6-phosphate receptor has gained an IGF-II-binding function and is termed type II IGF receptor (IGF2R). IGF2R sequesters IGF-II; imbalances occur in cancers and IGF2R is implicated in tumour suppression. We report crystal structures of IGF2R domains 11-12, 11-12-13-14 and domains 11-12-13/IGF-II complex. A distinctive juxtaposition of these domains provides the IGF-II-binding unit, with domain 11 directly interacting with IGF-II and domain 13 modulating binding site flexibility. Our complex shows that Phe19 and Leu53 of IGF-II lock into a hydrophobic pocket unique to domain 11 of mammalian IGF2Rs. Mutagenesis analyses confirm this IGF-II 'binding-hotspot', revealing that IGF-binding proteins and IGF2R have converged on the same high-affinity site.
About this Structure
2V5P is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure and functional analysis of the IGF-II/IGF2R interaction., Brown J, Delaine C, Zaccheo OJ, Siebold C, Gilbert RJ, van Boxel G, Denley A, Wallace JC, Hassan AB, Forbes BE, Jones EY, EMBO J. 2008 Jan 9;27(1):265-76. Epub 2007 Nov 29. PMID:18046459
Page seeded by OCA on Mon Mar 31 05:08:33 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Homo sapiens
- Protein complex
- Boxel, G Van.
- Brown, J.
- Delaine, C.
- Denley, A.
- Forbes, B E.
- Gilbert, R J.
- Hassan, A B.
- Jones, E Y.
- Siebold, C.
- Wallace, J C.
- Zaccheo, O J.
- Beta barrel
- Cation independent mannose 6-phosphate
- Fibronectin type ii
- Glycoprotein
- Insulin-like growth factor
- Lysosome
- Membrane
- Phosphorylation
- Polymorphism
- Receptor
- Receptor/glycoprotein complex
- Transmembrane
- Transport
