6gxt | pdb_00006gxt

The hit-and-return system enables efficient time-resolved serial synchrotron crystallography: FAcD2052MS after reaction initiation

spinqualitylabelsall models 6gxt, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6gxt is a 2 chain structure with sequence from Rhopa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FAH Gene: RPA1163 (RHOPA) Activity: Haloacetate dehalogenase, with EC number 3.8.1.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DEHA_RHOPA] Catalyzes the hydrolytic defluorination of fluoroacetate to produce glycolate. Has lower activity towards bromoacetate and chloroacetate. Publication Abstract from PubMed We present a 'hit-and-return' (HARE) method for time-resolved serial synchrotron crystallography with time resolution from milliseconds to seconds or longer. Timing delays are set mechanically, using the regular pattern in fixed-target crystallography chips and a translation stage system. Optical pump-probe experiments to capture intermediate structures of fluoroacetate dehalogenase binding to its ligand demonstrated that data can be collected at short (30 ms), medium (752 ms) and long (2,052 ms) intervals. The hit-and-return system enables efficient time-resolved serial synchrotron crystallography.,Schulz EC, Mehrabi P, Muller-Werkmeister HM, Tellkamp F, Jha A, Stuart W, Persch E, De Gasparo R, Diederich F, Pai EF, Miller RJD Nat Methods. 2018 Nov;15(11):901-904. doi: 10.1038/s41592-018-0180-2. Epub 2018, Oct 30. PMID:30377366 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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