6e65 | pdb_00006e65

Crystal structure of malaria transmission-blocking antigen Pfs48/45 6C in complex with antibody 85RF45.1

spinqualitylabelsall models 6e65, resolution 1.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6e65 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Interventions that can block the transmission of malaria-causing Plasmodium falciparum (Pf) between the human host and Anopheles vector have the potential to reduce the incidence of malaria. Pfs48/45 is a gametocyte surface protein critical for parasite development and transmission, and its targeting by monoclonal antibody (mAb) 85RF45.1 leads to the potent reduction of parasite transmission. Here, we reveal how the Pfs48/45 6C domain adopts a (SAG1)-related-sequence (SRS) fold. We structurally delineate potent epitope I and show how mAb 85RF45.1 recognizes an electronegative surface with nanomolar affinity. Analysis of Pfs48/45 sequences reveals that polymorphisms are rare for residues involved at the binding interface. Humanization of rat-derived mAb 85RF45.1 conserved the mode of recognition and activity of the parental antibody, while also improving its thermostability. Our work has implications for the development of transmission-blocking interventions, both through improving vaccine designs and the testing of passive delivery of mAbs in humans. Structural delineation of potent transmission-blocking epitope I on malaria antigen Pfs48/45.,Kundu P, Semesi A, Jore MM, Morin MJ, Price VL, Liang A, Li J, Miura K, Sauerwein RW, King CR, Julien JP Nat Commun. 2018 Oct 26;9(1):4458. doi: 10.1038/s41467-018-06742-9. PMID:30367064 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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