6dlh | pdb_00006dlh

Endo-fucoidan hydrolase MfFcnA4 from glycoside hydrolase family 107

spinqualitylabelsall models 6dlh, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6dlh is a 1 chain structure with sequence from Cip 109502. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, EDO, NA Gene: fcnA (CIP 109502) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Fucoidans are chemically complex and highly heterogeneous sulfated marine fucans from brown macro algae. Possessing a variety of physicochemical and biological activities, fucoidans are used as gelling and thickening agents in the food industry, and have anticoagulant, antiviral, antitumor, antibacterial, and immune activities. Although fucoidan-depolymerizing enzymes have been identified, the molecular basis of their activity on these chemically complex polysaccharides remains largely uninvestigated. In this study, we focused on three glycoside hydrolase family 107 (GH107) enzymes, MfFcnA and two newly identified members, P5AFcnA and P19DFcnA, from a bacterial species of the genus Psychromonas. Using carbohydrate-polyacrylamide gel electrophoresis, we show that P5AFcnA and P19DFcnA are active on fucoidans that differ from those depolymerized by MfFcnA, revealing differential substrate specificity within the GH107 family. Using a combination of X-ray crystallography and NMR analyses, we further show that GH107 family enzymes share features of their structures and catalytic mechanisms with GH29 alpha-L-fucosidases. However, we found that GH107 enzymes have the distinction of utilizing a histidine sidechain as the proposed acid/base catalyst in its retaining mechanism. Further interpretation of the structural data indicated that the active-site architectures within this family are highly variable, likely reflecting the specificity of GH107 enzymes for different fucoidan substructures. Together, these findings begin to illuminate the molecular details underpinning the biological processing of fucoidans. Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to alpha-L-fucosidases from GH29.,Vickers C, Liu F, Abe K, Salama-Alber O, Jenkins ML, Springate CM, Burke JE, Withers SG, Boraston AB J Biol Chem. 2018 Oct 3. pii: RA118.005134. doi: 10.1074/jbc.RA118.005134. PMID:30282808 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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