3era | pdb_00003era

RECOMBINANT ERABUTOXIN A (S8T MUTANT)

spinqualitylabelsall models 3era, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3era is a 2 chain structure with sequence from Black-banded sea krait. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: SCN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [NXSA_PSSEM] Binds with high affinity to muscular nicotinic acetylcholine receptors (nAChRs) and with low affinity to neuronal alpha-7 nAChRs and inhibit acetylcholine from binding to the receptor, thereby impairing neuromuscular transmission. Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Blocks the extracellular increase of dopamine evoked by nicotine at 4.2 uM concentrations. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed A previous mutational analysis of erabutoxin a (Ea), a curaremimetic toxin from sea snake venom, showed that the substitutions S8G and S8T caused, respectively, 176-fold and 780-fold affinity decreases for the nicotinic acetylcholine receptor (AchR). In view of the fact that the side-chain of Ser8 is buried in the wild-type toxin, we wondered whether these affinity changes reflect a direct binding contribution of S8 to the receptor and/or conformational changes that could have occurred in Ea as a result of the introduced mutations. To approach this question, we solved X-ray structures of the two mutants S8G and S8T at high resolution (0.18 nm and 0.17 nm, with R factors of 18.0% and 17.9%, respectively). The data show that none of the mutations significantly modified the toxin structure. Even within the site where the toxin binds to the receptor the backbone conformation remained unchanged. Therefore, the low affinities of the mutants S8T and S8G cannot be explained by a large conformational change of the toxin structure. Although we cannot exclude the possibility that undetectable structural changes have occurred in the toxin mutants, our data support the view that, although buried between loop I and II, S8 is part of the functional epitope of the toxin. High resolution x-ray analysis of two mutants of a curaremimetic snake toxin.,Gaucher JF, Menez R, Arnoux B, Pusset J, Ducruix A Eur J Biochem. 2000 Mar;267(5):1323-9. PMID:10691969 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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