3ndr | pdb_00003ndr

Crystal structure of tetrameric pyridoxal 4-dehydrogenase from Mesorhizobium loti

spinqualitylabelsall models 3ndr, resolution 2.88Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3ndr is a 4 chain structure with sequence from Atcc 700743. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NAD NonStd Res: MSE Gene: mlr6807, pldh-t (ATCC 700743) Activity: Pyridoxal 4-dehydrogenase, with EC number 1.1.1.107 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PLDH_RHILO] Involved in the degradation of pyridoxine or pyridoxamine (free, phosphate-unbound, forms of vitamin B6). Oxidizes pyridoxal to 4-pyridoxolactone, but does not have activity toward pyridoxal 5'-phosphate, pyridoxine, pyridoxamine, pyridoxamine 5'-phosphate, 4-phthalaldehyde, 2-nitrobenzaldehyde, pyridine, formaldehyde, 2-carboxybenzaldehyde or sugars. Publication Abstract from PubMed Pyridoxal 4-dehydrogenase from Mesorhizobium loti MAFF303099 was overexpressed in Escherichia coli. The recombinant selenomethionine-substituted enzyme was purified and crystallized by the sitting-drop vapour-diffusion method using PEG 4000 as precipitant. Crystals grew in the presence of 0.45 mM NAD(+). The crystals diffracted to 2.9 A resolution and belonged to the monoclinic space group P2(1), with unit-cell parameters a = 86.20, b = 51.11, c = 91.73 A, beta = 89.36 degrees. The calculated V(M) values suggested that the asymmetric unit contained four molecules. Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti.,Chu HN, Kobayashi J, Yoshikane Y, Mikami B, Yagi T Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jun 1;66(Pt, 6):718-20. Epub 2010 May 29. PMID:20516609 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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