4kf5 | pdb_00004kf5

Crystal Structure of Split GFP complexed with engineered sfCherry with an insertion of GFP fragment

spinqualitylabelsall models 4kf5, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4kf5 is a 4 chain structure with sequence from Synthetic construct sequences. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. NonStd Res: CH6, CRO Related: 4kf4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed A modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP beta-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10-11) hairpin in complex with GFP(1-9) was determined at a resolution of 2.6 A. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10-11) hairpin with a variety of GFP(1-9) mutants engineered for favorable crystallization. Split green fluorescent protein as a modular binding partner for protein crystallization.,Nguyen HB, Hung LW, Yeates TO, Terwilliger TC, Waldo GS Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2513-23. doi:, 10.1107/S0907444913024608. Epub 2013 Nov 19. PMID:24311592 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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