6j8w | pdb_00006j8w

Structure of MOEN5-SSO7D fusion protein in complex with lig 1

spinqualitylabelsall models 6j8w, resolution 2.35Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6j8w is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: C0X Related: 5gwv Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The antibiotic moenomycin A is a phosphoglycerate derivative with a C25-moenocinyl chain and a branched oligosaccharide. Formation of the C25-chain is catalyzed by the enzyme MoeN5 with geranyl pyrophosphate (GPP) and the sugar-linked 2-Z,E-farnesyl-3-phosphoglycerate (FPG) as its substrates. Previous complex crystal structures with GPP and long-chain alkyl glycosides suggested that GPP binds to the S1 site in a similar way as in most other alpha-helical prenyltransferases (PTs), and FPG is likely to assume a bent conformation in the S2 site. However, two FPG derivatives synthesized in the current study were found in the S1 site rather than S2 in their complex crystal structures with MoeN5. Apparently S1 is the preferred site for prenyl-containing ligand, and S2 binding may proceed only after S1 is occupied. Thus, like most trans-type PTs, MoeN5 may employ a sequential ionization-condensation-elimination mechanism that involves a carbocation intermediate. Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism.,Zhang L, Ko TP, Malwal SR, Liu W, Zhou S, Yu X, Oldfield E, Guo RT, Chen CC Biochem Biophys Res Commun. 2019 Apr 16;511(4):800-805. doi:, 10.1016/j.bbrc.2019.02.131. Epub 2019 Mar 2. PMID:30837154 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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