5krp | pdb_00005krp

Frutapin, a lectin from Artocarpus incisa: Cloning, Expressing and Structural Analysis.

spinqualitylabelsall models 5krp, resolution 1.58Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5krp is a 4 chain structure with sequence from Artal. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Artocarpus incisa (breadfruit) seeds contain three different lectins (Frutalin, Frutapin (FTP) and Frutackin) with distinct carbohydrate specificities. The most abundant lectin is Frutalin, an alpha-D-galactose-specific carbohydrate-binding glycoprotein with antitumour properties and potential for tumour biomarker discovery as already reported. FTP is the second most abundant, but proved difficult to purify with very low yields and contamination with Frutalin frustrating its characterization. Here, we report for the first time high-level production and isolation of biologically active recombinant FTP in Escherichia coli BL21, optimizing conditions with the best set yielding >40 mg/l culture of soluble active FTP. The minimal concentration for agglutination of red blood cells was 62.5 microg/ml of FTP, a process effectively inhibited by mannose. Apo-FTP, FTP-mannose and FTP-glucose crystals were obtained, and they diffracted X-rays to a resolution of 1.58 (P212121), 1.70 (P3121) and 1.60 (P3121) A respectively. The best solution showed four monomers per asymmetric unit. Molecular dynamics (MD) simulation suggested that FTP displays higher affinity for mannose than glucose. Cell studies revealed that FTP was non-cytotoxic to cultured mouse fibroblast 3T3 cells below 0.5 mg/ml and was also capable of stimulating cell migration at 50 microg/ml. In conclusion, our optimized expression system allowed high amounts of correctly folded soluble FTP to be isolated. This recombinant bioactive lectin will now be tested in future studies for therapeutic potential; for example in wound healing and tissue regeneration. Frutapin, a lectin from Artocarpus incisa (breadfruit): cloning, expression and molecular insights.,de Sousa FD, da Silva BB, Furtado GP, Carneiro IS, Lobo MDP, Guan Y, Guo J, Coker AR, Lourenzoni MR, Guedes MIF, Owen JS, Abraham DJ, Monteiro-Moreira ACO, Moreira RA Biosci Rep. 2017 Jul 21;37(4). pii: BSR20170969. doi: 10.1042/BSR20170969. Print , 2017 Aug 31. PMID:28684550 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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