4pi2 | pdb_00004pi2

Crystal structure of particulate methane monooxygenase from Methylocystis sp. ATCC 49242 (Rockwell) soaked in zinc

spinqualitylabelsall models 4pi2, resolution 3.33Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4pi2 is a 12 chain structure with sequence from Methylocystis sp. atcc 49242. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CAC, CU, ZN NonStd Res: UNK Related: 4phz, 4pi0 Activity: Methane monooxygenase (particulate), with EC number 1.14.18.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Particulate methane monooxygenase (pMMO) is a membrane-bound metalloenzyme that oxidizes methane to methanol in methanotrophic bacteria. Zinc is a known inhibitor of pMMO, but the details of zinc binding and the mechanism of inhibition are not understood. Metal binding and activity assays on membrane-bound pMMO from Methylococcus capsulatus (Bath) reveal that zinc inhibits pMMO at two sites that are distinct from the copper active site. The 2.6 A resolution crystal structure of Methylocystis species strain Rockwell pMMO reveals two previously undetected bound lipids, and metal soaking experiments identify likely locations for the two zinc inhibition sites. The first is the crystallographic zinc site in the pmoC subunit, and zinc binding here leads to ordering of 10 previously unobserved residues. A second zinc site is present on the cytoplasmic side of the pmoC subunit. Parallels between these results and zinc inhibition studies of several respiratory complexes suggest that zinc might inhibit proton transfer in pMMO. Effects of Zinc on Particulate Methane Monooxygenase Activity and Structure.,Sirajuddin S, Barupala D, Helling S, Marcus K, Stemmler TL, Rosenzweig AC J Biol Chem. 2014 Jun 18. pii: jbc.M114.581363. PMID:24942740 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Methane monooxygenase

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