6j4n | pdb_00006j4n

Structure of papua new guinea MBL-1(PNGM-1) native

spinqualitylabelsall models 6j4n, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6j4n is a 8 chain structure with sequence from Aciba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Metallo-beta-lactamases (MBLs) are present in major Gram-negative pathogens and environmental species, and pose great health risks because of their ability to hydrolyze the beta-lactam rings of antibiotics such as carbapenems. PNGM-1 was the first reported case of a subclass B3 MBL protein that was identified from a metagenomic library from deep-sea sediments that predate the antibiotic era. In this study, PNGM-1 was overexpressed, purified and crystallized. Crystals of native and selenomethionine-substituted PNGM-1 diffracted to 2.10 and 2.30 A resolution, respectively. Both the native and the selenomethionine-labelled PNGM-1 crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 122, b = 83, c = 163 A, beta = 110 degrees . Matthews coefficient (VM) calculations suggested the presence of 6-10 molecules in the asymmetric unit, corresponding to a solvent content of approximately 31-58%. Structure determination is currently in progress. The novel metallo-beta-lactamase PNGM-1 from a deep-sea sediment metagenome: crystallization and X-ray crystallographic analysis.,Park KS, Hong MK, Jeon JW, Kim JH, Jeon JH, Lee JH, Kim TY, Karim AM, Malik SK, Kang LW, Lee SH Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):644-649. doi:, 10.1107/S2053230X18012268. Epub 2018 Sep 19. PMID:30279316 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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