4v96 | pdb_00004v96

The structure of a 1.8 MDa viral genome injection device suggests alternative infection mechanisms

spinqualitylabelsall models 4v96, resolution 3.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4v96 is a 78 chain structure with sequence from Bacteriophage tp901-1. This structure supersedes the now removed PDB entries 4div and 4diw. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3uh8, 3u6x Gene: BppU (ORF48) (Bacteriophage TP901-1), Dit (ORF46) (Bacteriophage TP901-1), bpp, BppL (ORF49) (Bacteriophage TP901-1) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Phages of the Caudovirales order possess a tail that recognizes the host and ensures genome delivery upon infection. The X-ray structure of the approximately 1.8 MDa host adsorption device (baseplate) from the lactococcal phage TP901-1 shows that the receptor-binding proteins are pointing in the direction of the host, suggesting that this organelle is in a conformation ready for host adhesion. This result is in marked contrast with the lactococcal phage p2 situation, whose baseplate is known to undergo huge conformational changes in the presence of Ca(2+) to reach its active state. In vivo infection experiments confirmed these structural observations by demonstrating that Ca(2+) ions are required for host adhesion among p2-like phages (936-species) but have no influence on TP901-1-like phages (P335-species). These data suggest that these two families rely on diverse adhesion strategies which may lead to different signaling for genome release. Structure of the phage TP901-1 1.8 MDa baseplate suggests an alternative host adhesion mechanism.,Veesler D, Spinelli S, Mahony J, Lichiere J, Blangy S, Bricogne G, Legrand P, Ortiz-Lombardia M, Campanacci V, van Sinderen D, Cambillau C Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8954-8. Epub 2012 May 18. PMID:22611190 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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