6bnz | pdb_00006bnz

Crystal structure of E144Q-glyoxalase I mutant from Zea mays in space group P4(1)2(1)2

spinqualitylabelsall models 6bnz, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6bnz is a 1 chain structure with sequence from Maize. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CO, FMT, GSH Related: 5d7z, 6bnn Activity: Lactoylglutathione lyase, with EC number 4.4.1.5 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [B6TPH0_MAIZE] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.[RuleBase:RU361179] Publication Abstract from PubMed Detoxification of methylglyoxal, a toxic by-product of central sugar metabolism, is a major issue for all forms of life. The glyoxalase pathway evolved to effectively convert methylglyoxal into d-lactate via a glutathione hemithioacetal intermediate. Recently, we have shown that the monomeric glyoxalase I from maize exhibits a symmetric fold with two cavities, potentially harboring two active sites, in analogy with homodimeric enzyme surrogates. Here we confirm that only one of the two cavities exhibits glyoxalase I activity and show that it adopts a tunnel-shaped structure upon substrate binding. Such conformational change gives rise to independent binding sites for glutathione and methylglyoxal in the same active site, with important implications for the molecular reaction mechanism, which has been a matter of debate for several decades. DATABASE: Structural data are available in The Protein Data Bank database under the accession numbers 6BNN, 6BNX, and 6BNZ. Deciphering the number and location of active sites in the monomeric glyoxalase I of Zea mays.,Gonzalez JM, Agostini RB, Alvarez CE, Klinke S, Andreo CS, Campos-Bermudez VA FEBS J. 2019 Apr 16. doi: 10.1111/febs.14855. PMID:30993890 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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