6jg9 | pdb_00006jg9
Crystal structure of AimR in complex with arbitrium peptide
Structural highlights
Function[AIMR_BPSPB] Transcriptional regulator which is part of the latency-replication switch system that decides at the onset of infection whether to replicate and lyse the host or to lysogenize (latency) and keep the host viable. Activates the transcription of the aimX locus. Transcriptional activation of aimX seems to lead to the productive viral replication (lytic cycle), aimX possibly acting as a regulatory non-coding RNA.[UniProtKB:P0DOE3] Publication Abstract from PubMedA newly identified arbitrium communication system regulates the lysis-to-lysogeny decision in a Bacillus bacteriophage. This system contains an arbitrium hexapeptide as a signal, the cellular receptor AimR, and the lysogenic negative regulator AimX. AimR specifically targets the downstream DNA to activate aimX gene expression. The arbitrium peptide binds to AimR, inhibiting its DNA-binding to promote phage lysogeny. Recently, we and other groups have elucidated how arbitrium peptide sensed by AimR. However, the molecular mechanisms of DNA recognition by AimR and the regulation of its DNA-binding activity by the peptide remain largely unknown. Here, we report the crystal structure of the AimR-DNA complex at 2.1 A resolution. The N-terminal HTH motif recognizes the palindromic DNA sequence, buttressed by interactions between positively charged residues and the DNA phosphate groups. The DNA-bound AimR assembles a more closed dimer than the peptide-bound form. Single-molecule FRET and crosslinking assays revealed that the AimR protein samples both open and closed conformations in solution. Arbitrium peptide binding induces a closed-to-open conformational change of AimR, eliminating DNA targeting. Our structural and functional analysis provides new insights into the DNA recognition mechanism of AimR and its regulation by the arbitrium peptide in the context of phage lysis-lysogeny decisions. Structural insights into DNA recognition by AimR of the arbitrium communication system in the SPbeta phage.,Guan Z, Pei K, Wang J, Cui Y, Zhu X, Su X, Zhou Y, Zhang D, Tang C, Yin P, Liu Z, Zou T Cell Discov. 2019 May 28;5:29. doi: 10.1038/s41421-019-0101-2. eCollection 2019. PMID:31149347[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
| ||||||||||||||||
