6reg | pdb_00006reg

Crystal structure of Pizza6-S with Zn2+

spinqualitylabelsall models 6reg, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6reg is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed We developed an artificial hydrolase based on the symmetrical Pizza6 beta-propeller protein for the metal-free hydrolysis of 4-nitrophenyl acetate and butyrate. Through site-specific mutagenesis and crystallisation studies, the catalytic mechanism was investigated and found to be dependent on a threonine-histidine dyad. The mutant with additional histidine residues generated the highest kcat values, forming a His-His-Thr triad and matched previously reported metalloenzymes. The highly symmetrical beta-propeller artificial enzymes and their protein-metal complexes have potential to be utilised in bioinorganic and supramolecular chemistry, as well as being developed further into 2D/3D catalytic materials. Artificial beta-propeller protein-based hydrolases.,Clarke DE, Noguchi H, Gryspeerdt JAG, De Feyter S, Voet ARD Chem Commun (Camb). 2019 Jul 23;55(60):8880-8883. doi: 10.1039/c9cc04388h. PMID:31321399 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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