6hg6 | pdb_00006hg6

Clostridium beijerinckii aldo-keto reductase Cbei_3974 with NADPH

spinqualitylabelsall models 6hg6, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6hg6 is a 1 chain structure with sequence from "clostridium_rubrum"_ng_and_vaughn_1963 "clostridium rubrum" ng and vaughn 1963. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: EDO, NDP Gene: gpr_2, CBEIJ_11710 ("Clostridium rubrum" Ng and Vaughn 1963) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Many aldehydes such as furfural are present in high quantities in lignocellulose lysates and are fermentation inhibitors that make biofuel production from this abundant carbon source extremely challenging. Cbei_3974 has recently been identified as an aldo-keto reductase responsible for partial furfural resistance in Clostridium beijerinkii Rational engineering of this enzyme could enhance the furfural tolerance of this organism thereby improving biofuel yields. We report an extensive characterization of Cbei_3974 and a single crystal X-ray structure of Cbei_3974 in complex with NADPH at a resolution of 1.75 A. Docking studies identified residues involved in substrate binding and an activity screen revealed the substrate tolerance of the enzyme. Hydride transfer, which is partially rate limiting under physiological conditions, occurs from the pro-R hydrogen of NADPH. Enzyme isotope labeling revealed a temperature-independent enzyme isotope effect of unity, indicating that the enzyme does not use dynamic coupling for catalysis and suggests that the active site of the enzyme is optimally configured for catalysis with the substrate tested.ImportanceHerein is reported the crystal structure and biophysical properties of an aldehyde reductase that can detoxify furfural, a common inhibitor of biofuel fermentation found in lignocellulose lysates. The data contained herein will serve as a guide for protein engineers to develop improved enzyme variants that would infer furfural resistance to the microorganisms used in biofuel production and thus lead to enhanced biofuel yields from this sustainable resource. Crystal Structure and Biophysical Analysis of Furfural Detoxifying Aldehyde Reductase from Clostridium beijerinkii.,Scott AF, Cresser-Brown J, Williams TL, Rizkallah PJ, Jin Y, Luk LY, Allemann RK Appl Environ Microbiol. 2019 May 17. pii: AEM.00978-19. doi:, 10.1128/AEM.00978-19. PMID:31101612 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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