6k3l | pdb_00006k3l
Crystal structure of CX-4945 bound Cka1 from C. neoformans
Structural highlights
Publication Abstract from PubMedCK2alpha is a constitutively active and highly conserved serine/threonine protein kinase that is involved in the regulation of key cellular metabolic pathways and associated with a variety of tumours and cancers. The most well-known CK2alpha inhibitor is the human clinical trial candidate CX-4945, which has recently shown to exhibit not only anti-cancer, but also anti-fungal properties. This prompted us to work on the CK2alpha orthologue, Cka1, from the pathogenic fungus Cryptococcus neoformans, which causes life-threatening systemic cryptococcosis and meningoencephalitis mainly in immunocompromised individuals. At present, treatment of cryptococcosis remains a challenge due to limited anti-cryptococcal therapeutic strategies. Hence, expanding therapeutic options for the treatment of the disease is highly clinically relevant. Herein, we report the structures of Cka1-AMPPNP-Mg(2+) (2.40 A) and Cka1-CX-4945 (2.09 A). Structural comparisons of Cka1-AMPPNP-Mg(2+) with other orthologues revealed the dynamic architecture of the N-lobe across species. This may explain for the difference in binding affinities and deviations in protein-inhibitor interactions between Cka1-CX-4945 and human CK2alpha-CX-4945. Supporting it, in vitro kinase assay demonstrated that CX-4945 inhibited human CK2alpha much more efficiently than Cka1. Our results provide structural insights into the design of more selective inhibitors against Cka1. Structural analysis of fungal pathogenicity-related casein kinase alpha subunit, Cka1, in the human fungal pathogen Cryptococcus neoformans.,Ong BX, Yoo Y, Han MG, Park JB, Choi MK, Choi Y, Shin JS, Bahn YS, Cho HS Sci Rep. 2019 Oct 7;9(1):14398. doi: 10.1038/s41598-019-50678-z. PMID:31591414[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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