6rls | pdb_00006rls

Concerted dynamics of metallo-base pairs in an A/B-form helical transition (apo species)

spinqualitylabelsall models 6rls, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6rls is a 2 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 6fy7, 6fy6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Metal-mediated base pairs expand the repertoire of nucleic acid structures and dynamics. Here we report solution structures and dynamics of duplex DNA containing two all-natural C-Hg(II)-T metallo base pairs separated by six canonical base pairs. NMR experiments reveal a 3:1 ratio of well-resolved structures in dynamic equilibrium. The major species contains two (N3)T-Hg(II)-(N3)C base pairs in a predominantly B-form helix. The minor species contains (N3)T-Hg(II)-(N4)C base pairs and greater A-form characteristics. Ten-fold different (1)J coupling constants ((15)N,(199)Hg) are observed for (N3)C-Hg(II) (114 Hz) versus (N4)C-Hg(II) (1052 Hz) connectivities, reflecting differences in cytosine ionization and metal-bonding strengths. Dynamic interconversion between the two types of C-Hg(II)-T base pairs are coupled to a global conformational exchange between the helices. These observations inspired the design of a repetitive DNA sequence capable of undergoing a global B-to-A-form helical transition upon adding Hg(II), demonstrating that C-Hg(II)-T has unique switching potential in DNA-based materials and devices. Concerted dynamics of metallo-base pairs in an A/B-form helical transition.,Schmidt OP, Jurt S, Johannsen S, Karimi A, Sigel RKO, Luedtke NW Nat Commun. 2019 Oct 23;10(1):4818. doi: 10.1038/s41467-019-12440-x. PMID:31645548 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA