5eqw | pdb_00005eqw

Structure of the major structural protein D135 of Acidianus tailed spindle virus (ATSV)

spinqualitylabelsall models 5eqw, resolution 1.68Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5eqw is a 5 chain structure with sequence from Acidianus tailed spindle virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NO3 NonStd Res: MSE Gene: ATSV_D135 (Acidianus tailed spindle virus) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The spindle-shaped virion morphology is common among archaeal viruses, where it is a defining characteristic of many viral families. However, structural heterogeneity intrinsic to spindle-shaped viruses has seriously hindered efforts to elucidate the molecular architecture of these lemon-shaped capsids. We have utilized a combination of cryo-electron microscopy and X-ray crystallography to study Acidianus tailed spindle virus (ATSV). These studies reveal the architectural principles that underlie assembly of a spindle-shaped virus. Cryo-electron tomography shows a smooth transition from the spindle-shaped capsid into the tubular-shaped tail and allows low-resolution structural modeling of individual virions. Remarkably, higher-dose 2D micrographs reveal a helical surface lattice in the spindle-shaped capsid. Consistent with this, crystallographic studies of the major capsid protein reveal a decorated four-helix bundle that packs within the crystal to form a four-start helical assembly with structural similarity to the tube-shaped tail structure of ATSV and other tailed, spindle-shaped viruses. Combined, this suggests that the spindle-shaped morphology of the ATSV capsid is formed by a multistart helical assembly with a smoothly varying radius and allows construction of a pseudoatomic model for the lemon-shaped capsid that extends into a tubular tail. The potential advantages that this novel architecture conveys to the life cycle of spindle-shaped viruses, including a role in DNA ejection, are discussed. Structural studies of Acidianus tailed spindle virus reveal a structural paradigm used in the assembly of spindle-shaped viruses.,Hochstein R, Bollschweiler D, Dharmavaram S, Lintner NG, Plitzko JM, Bruinsma R, Engelhardt H, Young MJ, Klug WS, Lawrence CM Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2120-2125. doi:, 10.1073/pnas.1719180115. Epub 2018 Feb 12. PMID:29440399 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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