6pw1 | pdb_00006pw1

Cytochrome c Oxidase delta 16

spinqualitylabelsall models 6pw1, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6pw1 is a 4 chain structure with sequence from Rhos4. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA, CD, CU, DMU, HEA, HTH, MAL, MG, TRD, TRS Related: 2gsm Gene: coxI, RSP_1877 (RHOS4), coxII, RSP_1826 (RHOS4) Activity: Cytochrome-c oxidase, with EC number 1.9.3.1 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [Q3J5G0_RHOS4] Subunits I and II form the functional core of the enzyme complex. Electrons originating in cytochrome c are transferred via heme a and Cu(A) to the binuclear center formed by heme a3 and Cu(B).[RuleBase:RU004024] Publication Abstract from PubMed Data from earlier studies showed that minor structural changes at the surface of cytochrome c oxidase, near one of the proton-input pathways (the D pathway), result in dramatically decreased activity and a lower proton-pumping stoichiometry. To further investigate how changes around the D pathway orifice influence functionality of the enzyme, here we modified the nearby C-terminal loop of subunit I of the Rhodobacter sphaeroides cytochrome c oxidase. Removal of 16 residues form this flexible surface loop resulted in a decrease in the proton-pumping stoichiometry to <50% of that of the wild-type enzyme. Replacement of the protonatable residue Glu552, part of the same loop, by an Ala, resulted in a similar decrease in the proton-pumping stoichiometry without loss of the O2-reduction activity or changes in the proton-uptake kinetics. The data show that minor structural changes at the orifice of the D pathway, at a distance of ~40A from the proton gate of cytochrome c oxidase, may alter the proton-pumping stoichiometry of the enzyme. Structural changes at the surface of cytochrome c oxidase alter the proton-pumping stoichiometry.,Berg J, Liu J, Svahn E, Ferguson-Miller S, Brzezinski P Biochim Biophys Acta Bioenerg. 2019 Nov 13:148116. doi:, 10.1016/j.bbabio.2019.148116. PMID:31733183 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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