6pkm | pdb_00006pkm

MicroED structure of proteinase K from an uncoated, single lamella at 2.17A resolution (#8)

spinqualitylabelsall models 6pkm, resolution 2.17Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6pkm is a 1 chain structure with sequence from Parengyodontium album. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Activity: Peptidase K, with EC number 3.4.21.64 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [PRTK_PARAQ] Hydrolyzes keratin at aromatic and hydrophobic residues. Publication Abstract from PubMed Microcrystal electron diffraction (MicroED) leverages the strong interaction between matter and electrons to determine protein structures from vanishingly small crystals. This strong interaction limits the thickness of crystals that can be investigated by MicroED, mainly due to absorption. Recent studies have demonstrated that focused ion-beam (FIB) milling can thin crystals into ideal-sized lamellae; however, it is not clear how to best apply FIB milling for MicroED. Here, the effects of polishing the lamellae, whereby the last few nanometers are milled away using a low-current gallium beam, are explored in both the platinum-precoated and uncoated samples. Our results suggest that precoating samples with a thin layer of platinum followed by polishing the crystal surfaces prior to data collection consistently led to superior results as indicated by higher signal-to-noise ratio, higher resolution, and better refinement statistics. This study lays the foundation for routine and reproducible methodology for sample preparation in MicroED. Qualitative Analyses of Polishing and Precoating FIB Milled Crystals for MicroED.,Martynowycz MW, Zhao W, Hattne J, Jensen GJ, Gonen T Structure. 2019 Oct 1;27(10):1594-1600.e2. doi: 10.1016/j.str.2019.07.004. Epub, 2019 Aug 15. PMID:31422911 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Proteinase Proteinase 3D structures

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