6ne8 | pdb_00006ne8

Solution Structure of the Thioredoxin-like Domain of Arabidopsis NCP (NUCLEAR CONTROL OF PEP ACTIVITY)

spinqualitylabelsall models 6ne8, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ne8 is a 1 chain structure. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [MRL7L_ARATH] Plays an essential role in early steps of chloroplast development (PubMed:21515910, PubMed:24111559). Involved in the regulation of plastid gene expression (PubMed:21515910, PubMed:24111559). Required for the proper function of the plastid transcriptional machinery and protein accumulation in thylakoid membranes. May function as molecular chaperone to ensure proper organization of the nucleoids in chloroplasts (PubMed:24111559). Publication Abstract from PubMed Phytochromes initiate chloroplast biogenesis by activating genes encoding the photosynthetic apparatus, including photosynthesis-associated plastid-encoded genes (PhAPGs). PhAPGs are transcribed by a bacterial-type RNA polymerase (PEP), but how phytochromes in the nucleus activate chloroplast gene expression remains enigmatic. We report here a forward genetic screen in Arabidopsis that identified NUCLEAR CONTROL OF PEP ACTIVITY (NCP) as a necessary component of phytochrome signaling for PhAPG activation. NCP is dual-targeted to plastids and the nucleus. While nuclear NCP mediates the degradation of two repressors of chloroplast biogenesis, PIF1 and PIF3, NCP in plastids promotes the assembly of the PEP complex for PhAPG transcription. NCP and its paralog RCB are non-catalytic thioredoxin-like proteins that diverged in seed plants to adopt nonredundant functions in phytochrome signaling. These results support a model in which phytochromes control PhAPG expression through light-dependent double nuclear and plastidial switches that are linked by evolutionarily conserved and dual-localized regulatory proteins. NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches.,Yang EJ, Yoo CY, Liu J, Wang H, Cao J, Li FW, Pryer KM, Sun TP, Weigel D, Zhou P, Chen M Nat Commun. 2019 Jun 14;10(1):2630. doi: 10.1038/s41467-019-10517-1. PMID:31201314 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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