6crm | pdb_00006crm

Crystal Structure of RecQ catalytic core from C. sakazakii bound to an unfolded G-quadruplex

spinqualitylabelsall models 6crm, resolution 2.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6crm is a 2 chain structure with sequence from Cros8. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ZN Gene: ESA_03738 (CROS8) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Homeostatic regulation of G-quadruplexes (G4s), four-stranded structures that can form in guanine-rich nucleic acids, requires G4 unwinding helicases. The mechanisms that mediate G4 unwinding remain unknown. We report the structure of a bacterial RecQ DNA helicase bound to resolved G4 DNA. Unexpectedly, a guanine base from the unwound G4 is sequestered within a guanine-specific binding pocket. Disruption of the pocket in RecQ blocks G4 unwinding, but not G4 binding or duplex DNA unwinding, indicating its essential role in structure-specific G4 resolution. A novel guanine-flipping and sequestration model that may be applicable to other G4-resolving helicases emerges from these studies. A guanine-flipping and sequestration mechanism for G-quadruplex unwinding by RecQ helicases.,Voter AF, Qiu Y, Tippana R, Myong S, Keck JL Nat Commun. 2018 Oct 10;9(1):4201. doi: 10.1038/s41467-018-06751-8. PMID:30305632 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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