6bym | pdb_00006bym

Crystal structure of the sterol-bound second StART domain of yeast Lam4

spinqualitylabelsall models 6bym, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6bym is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: HC3 Gene: LAM4, SCKG_4597 (ATCC 18824) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The StARkin superfamily comprises proteins with steroidogenic acute regulatory protein related lipid transfer (StART) domains that are implicated in intracellular, non-vesicular lipid transport. A new family of membrane-anchored StARkins was recently identified, including six members Lam1-Lam6 in the yeast Saccharomyces cerevisiae. Lam1-Lam4 are anchored to the endoplasmic reticulum (ER) membrane at sites where the ER is tethered to the plasma membrane, and proposed to be involved in sterol homeostasis in yeast. To understand better the biological roles of these proteins, we carried out a structure-function analysis of the second StARkin domain of Lam4, here termed Lam4S2. NMR experiments indicated that Lam4S2 binds undergoes specific conformational changes upon binding sterol, and fluorescence-based assays revealed that it catalyzes sterol transport between vesicle populations in vitro, exhibiting a preference for vesicles containing anionic lipids. Using such vesicles, we found that sterols are transported at a rate of ~50 molecules per Lam4S2 per minute. Crystal structures of Lam4S2, with and without bound sterol revealed a largely hydrophobic but surprisingly accessible sterol binding pocket, with the 3-OH group of the sterol oriented towards its base. Single or multiple alanine or aspartic acid replacements of conserved lysine residues in a basic patch on the surface of Lam4S2 near the likely sterol entry/egress site strongly attenuated sterol transport. Our results suggest that Lam4S2 engages anionic membranes via a basic surface patch, enabling 'head-first' entry of sterol into the binding pocket followed by partial closure of the entryway. Reversal of these steps enables sterol egress. Structural basis of sterol binding and transport by a yeast StARkin domain.,Jentsch JA, Kiburu I, Pandey K, Timme M, Ramlall T, Levkau B, Wu J, Eliezer D, Boudker O, Menon AK J Biol Chem. 2018 Feb 20. pii: RA118.001881. doi: 10.1074/jbc.RA118.001881. PMID:29463678 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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