2kb2 | pdb_00002kb2

BlrP1 BLUF

spinqualitylabelsall models 2kb2, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2kb2 is a 1 chain structure with sequence from Klep7. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: FMN Gene: KPN78578_15680, KPN_01598 (KLEP7) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed BLUF (sensor of Blue Light Using FAD) domains are a group of flavin-containing blue light photosensory domains from a variety of bacterial and algal proteins. While spectroscopic studies have indicated that these domains reorganize their interactions with an internally-bound chromophore upon illumination, it remains unclear how these are converted into structural and functional changes. To address this, we have solved the solution structure of the BLUF domain from Klebsiella pneumoniae BlrP1, a light-activated c-di-GMP phosphodiesterase which consists of a sensory BLUF and a catalytic EAL domain. Our dark state structure of the sensory domain shows that it adopts a standard BLUF domain fold followed by two C-terminal alpha-helices which adopt a novel orientation with respect to the rest of the domain. Comparison of NMR spectra acquired under dark and lit conditions suggests that residues throughout the BlrP1 BLUF domain undergo significant light-induced chemical shift changes, including sites clustered on the ?4?5 loop, ?5 strand and ?3?4 loop. Given that these changes were observed at several sites on the helical cap, over 15 ? from chromophore, our data suggest a long-range signal transduction process in BLUF domains. Structure and insight into blue light-induced changes in the BlrP1 BLUF domain.,Wu Q, Gardner K Biochemistry. 2009 Feb 3. PMID:19191473 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Phosphodiesterase 3D structures

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