6tob | pdb_00006tob

Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF

spinqualitylabelsall models 6tob, 20 NMR models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6tob is a 1 chain structure with sequence from "bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: mihF, ERS007672_05099, ERS007703_04035 ("Bacillus tuberculosis" (Zopf 1883) Klein 1884) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted to Actinobacteria and essential for survival of the human pathogen Mycobacterium tuberculosis. We show in vitro that DNA binding by mIHF strongly stabilizes the protein and increases its melting temperature. The structure obtained by Nuclear Magnetic Resonance (NMR) spectroscopy characterizes mIHF as a globular protein with a protruding alpha helix and a disordered N-terminus, similar to Streptomyces coelicolor IHF (sIHF). NMR revealed no residues of high flexibility, suggesting that mIHF is a rigid protein overall that does not undergo structural rearrangements. We show that mIHF only binds to double stranded DNA in solution, through two DNA binding sites (DBSs) similar to those identified in the x-ray structure of sIHF. According to Atomic Force Microscopy, mIHF is able to introduce left-handed loops of ca. 100 nm size ( approximately 300 bp) in supercoiled cosmids, thereby unwinding and relaxing the DNA. Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF.,Odermatt NT, Lelli M, Herrmann T, Abriata LA, Japaridze A, Voilquin H, Singh R, Piton J, Emsley L, Dietler G, Cole ST J Struct Biol. 2019 Dec 14:107434. doi: 10.1016/j.jsb.2019.107434. PMID:31846718 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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