6imm | pdb_00006imm

Cryo-EM structure of an alphavirus, Sindbis virus

Loading... Status: waiting for Mol* initialization Structural highlights 6imm is a 12 chain structure with sequence from Sindbis virus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: 8K6 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Alphaviruses are enveloped RNA viruses that contain several human pathogens. Due to intrinsic heterogeneity of alphavirus particles, a high resolution structure of the virion is currently lacking. Here we provide a 3.5 A cryo-EM structure of Sindbis virus, using block based reconstruction method that overcomes the heterogeneity problem. Our structural analysis identifies a number of conserved residues that play pivotal roles in the virus life cycle. We identify a hydrophobic pocket in the subdomain D of E2 protein that is stabilized by an unknown pocket factor near the viral membrane. Residues in the pocket are conserved in different alphaviruses. The pocket strengthens the interactions of the E1/E2 heterodimer and may facilitate virus assembly. Our study provides structural insights into alphaviruses that may inform the design of drugs and vaccines. Implication for alphavirus host-cell entry and assembly indicated by a 3.5A resolution cryo-EM structure.,Chen L, Wang M, Zhu D, Sun Z, Ma J, Wang J, Kong L, Wang S, Liu Z, Wei L, He Y, Wang J, Zhang X Nat Commun. 2018 Dec 14;9(1):5326. doi: 10.1038/s41467-018-07704-x. PMID:30552337 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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