3j25 | pdb_00003j25

Structural basis for TetM-mediated tetracycline resistance

Loading... Status: waiting for Mol* initialization Structural highlights 3j25 is a 1 chain structure with sequence from "enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GCP Gene: tetM, transposon TnFO1 ("Enterococcus proteiformis" Thiercelin and Jouhaud 1903) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [TET1_ENTFL] Abolishes the inhibitory effect of tetracyclin on protein synthesis by a non-covalent modification of the ribosomes. Publication Abstract from PubMed Ribosome protection proteins (RPPs) confer tetracycline resistance by binding to the ribosome and chasing the drug from its binding site. The current model for the mechanism of action of RPPs proposes that drug release is indirect and achieved via conformational changes within the drug-binding site induced upon binding of the RPP to the ribosome. Here we report a cryo-EM structure of the RPP TetM in complex with the 70S ribosome at 7.2-A resolution. The structure reveals the contacts of TetM with the ribosome, including interaction between the conserved and functionally critical C-terminal extension of TetM and the decoding center of the small subunit. Moreover, we observe direct interaction between domain IV of TetM and the tetracycline binding site and identify residues critical for conferring tetracycline resistance. A model is presented whereby TetM directly dislodges tetracycline to confer resistance. Structural basis for TetM-mediated tetracycline resistance.,Donhofer A, Franckenberg S, Wickles S, Berninghausen O, Beckmann R, Wilson DN Proc Natl Acad Sci U S A. 2012 Oct 1. PMID:23027944 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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