6bu9 | pdb_00006bu9

Drosophila Dicer-2 bound to blunt dsRNA

Loading... Status: waiting for Mol* initialization Structural highlights 6bu9 is a 3 chain structure with sequence from Drome. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: Dcr-2, Dcr-2-RA, CG6493, Dmel_CG6493 (DROME) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Invertebrates rely on Dicer to cleave viral double-stranded RNA (dsRNA), and Drosophila Dicer-2 distinguishes dsRNA substrates by their termini. Blunt termini promote processive cleavage, while 3' overhanging termini are cleaved distributively. To understand this discrimination, we used cryo-electron microscopy to solve structures of Drosophila Dicer-2 alone and in complex with blunt dsRNA. While the Platform-PAZ domains have been considered the only Dicer domains that bind dsRNA termini, unexpectedly, we found that the helicase domain is required for binding blunt, but not 3' overhanging, termini. We further showed that blunt dsRNA is locally unwound and threaded through the helicase domain in an ATP-dependent manner. Our studies reveal a previously unrecognized mechanism for optimizing antiviral defense and set the stage for discovery of helicase-dependent functions in other Dicers. Dicer uses distinct modules for recognizing dsRNA termini.,Sinha NK, Iwasa J, Shen PS, Bass BL Science. 2017 Dec 21. pii: science.aaq0921. doi: 10.1126/science.aaq0921. PMID:29269422 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Dicer

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