6qcc | pdb_00006qcc

Cryo-EM Atomic Structure of Broad Bean Stain Virus (BBSV)

Loading... Status: waiting for Mol* initialization Structural highlights 6qcc is a 2 chain structure with sequence from Broad bean stain virus. This structure supersedes the now removed PDB entry 5npx. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The Broad bean stain virus (BBSV) is a member of the genus Comovirus infecting Fabaceae. The virus is transmitted through seed and by plant weevils causing severe and widespread disease worldwide. BBSV has a bipartite, positive-sense, single-stranded RNA genome encapsidated in icosahedral particles. We present here the cryo-electron microscopy reconstruction of the BBSV and an atomic model of the capsid proteins refined at 3.22A resolution. We identified residues involved in RNA/capsid interactions revealing a unique RNA genome organization. Inspection of the small coat protein C-terminal domain highlights a maturation cleavage between Leu567 and Leu568 and interactions of the C-terminal stretch with neighbouring small coat proteins within the capsid pentameric turrets. These interactions previously proposed to play a key role in the assembly of the Cowpea mosaic virus suggest a common capsid assembly mechanism throughout all comovirus species. The cryo-electron microscopy structure of Broad Bean Stain Virus suggests a common capsid assembly mechanism among comoviruses.,Lecorre F, Lai-Kee-Him J, Blanc S, Zeddam JL, Trapani S, Bron P Virology. 2019 Apr;530:75-84. doi: 10.1016/j.virol.2019.02.009. Epub 2019 Feb 13. PMID:30782565 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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