6rxv | pdb_00006rxv

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B2

Loading... Status: waiting for Mol* initialization Structural highlights 6rxv is a 68 chain structure with sequence from Chatd and Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GTP, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [G0S3V7_CHATD] Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.[PIRNR:PIRNR006515] [PNO1_CHATD] Required for small ribosomal subunit (SSU) synthesis. Has a role in the processing of early nucleolar and late cytoplasmic pre-RNA species.[UniProtKB:Q99216] [UTP11_CHATD] Involved in nucleolar processing of pre-18S ribosomal RNA.[UniProtKB:P34247] [NOL10_CHATD] May be involved in rRNA-processing and ribosome biosynthesis.[UniProtKB:P48234] Publication Abstract from PubMed Eukaryotic ribosome biogenesis involves RNA folding and processing that depend on assembly factors and small nucleolar RNAs (snoRNAs). The 90S (SSU-processome) is the earliest pre-ribosome structurally analyzed, which was suggested to assemble stepwise along the growing pre-rRNA from 5' > 3', but this directionality may not be accurate. Here, by analyzing the structure of a series of 90S assembly intermediates from Chaetomium thermophilum, we discover a reverse order of 18S rRNA subdomain incorporation. Large parts of the 18S rRNA 3' and central domains assemble first into the 90S before the 5' domain is integrated. This final incorporation depends on a contact between a heterotrimer Enp2-Bfr2-Lcp5 recruited to the flexible 5' domain and Kre33, which reconstitutes the Kre33-Enp-Brf2-Lcp5 module on the compacted 90S. Keeping the 5' domain temporarily segregated from the 90S scaffold could provide extra time to complete the multifaceted 5' domain folding, which depends on a distinct set of snoRNAs and processing factors. Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.,Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E Mol Cell. 2019 Jul 15. pii: S1097-2765(19)30489-7. doi:, 10.1016/j.molcel.2019.06.032. PMID:31378463 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosome 3D structures

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